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KMID : 1094720120170051031
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 5 p.1031 ~ p.1040
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Isolation and antioxidant activity evaluation of two new phthalate derivatives from seahorse, Hippocampus Kuda Bleeler
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Qian Zhong-Ji
Kang Kyong-Hwa
Kim Se-Kwon
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Abstract
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Seahorse (Hippocampus Kuda Bleeler) has been used as traditional medicine for thousands of years, in Eastern Asia. In this study of the methanol extract of fresh Hippocampus Kuda, the new compounds 2-ethyldecyl 2-ethylundecyl phthalate (1), 2, 12-diethyl-11-methylhexadecyl 2-ethyl-11-methylhexadecylphthalate (2), along with a known Bis(2-ethylheptyl) phthalate (3) were isolated. They were tested for their antioxidant activities, including lipid peroxidation inhibitory activity, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, and cellular radicals; these can be detected using a fluorescence probe, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on mouse macrophages, RAW264.7 cell. Compound (2) exhibited the highest antioxidant activity and inhibitory intracellular ROS than another compounds (1, 3). Furthermore, MTT assay showed no cytotoxicity on mouse macrophages cell (RAW264.7) and human fetal lung fibroblast cell line (MRC-5). This antioxidant property depends on concentration and increasing with increased amount of the compound.
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KEYWORD
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hippocampus kuda bleeler, phthalate, antioxidant activity, free radical scavenging, reactive oxygen species (ROS)
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